Abstract:

The objective of this study was to screen effective prescription of chemical drug and Chinese herbal medicines against Toxoplasmosis. First, the inhibiting effects of sulfachloropyrazine-sodium (SPZ), sulfadiazine-sodium (SD), scutellariae and glycyrrhizae against Toxoplama gondii (T. gondii) were observed on the Ana-1 cells in vitro. Then mice infected intraperitoneally with tachyzoites of T. gondii were treated separately with each drug and a prescription of SPZ combined with scutellariae and glycyrrhiza on mouse model in vivo. Our results indicated that each of the four drugs could significantly inhibit the proliferation of T. gondii in vitro and could prolong the time of death in mice infected with T. gondii, but Glycyrrhizae, Scutellariae or SD used alone could not protect these mice from death. Mice treated with SPZ alone could have a survival rate of 40%, while SPZ combined with Glycyrrhizae and Scutellariae, the survival rate of mice could be up to 50% and the abdominal cavity of the mice were tachyzoite-free. The results of this study suggest that SPZ have significant role in vitro and in vivo anti-T. gondii, while SPZ was applied with Chinese medicine-Glycyrrhizae and Scutellariae, not only the therapeutic effects could be improved, but also the dose of chemotherapy could be lower to reducing the toxicity of drugs. It can be concluded that the compound of SPZ, Glycyrrhizae andScutellariae will be a better choice against animal Toxoplasmosis in the future.